Journal: Nature Communications

Article Title: An in-situ peptide-antibody self-assembly to block CD47 and CD24 signaling enhances macrophage-mediated phagocytosis and anti-tumor immune responses

doi: 10.1038/s41467-024-49825-6

Figure Lengend Snippet: a Schematic illustration of tumor immune quiescent microenvironment, in situ assembly of PAC-SABIs on the cancer cell surface, and blockage of CD47 and CD24 phagocytic checkpoints by PAC-SABIs. Figure was created with BioRender.com and released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 international license. b Time-dependent CLSM images of 4T1 cells treated with NBD-labeled PAC-SABIs. Scale bar: 20 μm. Three independent experiments were performed. c Time-dependent CLSM images of PAN02 cells treated with NBD-labeled PAC-SABIs. Scale bar: 20 μm. Three independent experiments were performed. d Merged bright field and fluorescence images of 4T1 cell treated with NBD-labeled PAC-SABIs for 120 min. Scale bar: 20 μm. Three independent experiments were performed. e CLSM images of 4T1 cell treated with Dil dye (red) and NBD-labeled PAC-SABIs for 120 min. Scale bar: 10 μm. Three independent experiments were performed. f , g Fluorescence distribution of NBD-labeled PAC-SABIs on 4T1 cell. Three independent experiments were performed. h Time-dependent SEM images of 4T1 cells treated with PAC-SABIs. The red arrows point to PAC-SABIs on cell membrane. Scale bar: 2 μm. Three independent experiments were performed. i Time-dependent SEM images of PAN02 cells treated with PAC-SABIs. The red arrows point to the PAC-SABIs on cell membrane. Scale bar: 2 μm. Three independent experiments were performed. j CLSM images of 3D 4T1 spheroids treated with Cy5.5 PAC-SABIs, Calcein-AM, and Hoechst 33342. Scale bar: 50 μm. Three independent experiments were performed. k CLSM images of 3D 4T1 spheroids along the z -axis position. Scale bar: 50 μm. Three independent experiments were performed.

Article Snippet: Treatment: anti-mouse CD24 antibody (BioXcell, #BE0360, clone M1/69), anti-human CD24 antibody (Novus Biologics, #NB100-64861, clone SN3), anti-mouse CD47 antibody (BioXcell, #BE0270, clone MIAP301), anti-mouse PD-1 antibody (BioXcell, #BE0273, clone 29F.1A12TM), anti-CSF1R antibody (BioXcell, #BE0213, clone AFS98).

Techniques: In Situ, Labeling, Fluorescence, Membrane

Journal: Nature Communications

Article Title: An in-situ peptide-antibody self-assembly to block CD47 and CD24 signaling enhances macrophage-mediated phagocytosis and anti-tumor immune responses

doi: 10.1038/s41467-024-49825-6

Figure Lengend Snippet: a Phagocytosis images of pHrodo-red + over time. Scale bar: 50 μm. Three independent experiments were performed. b Phagocytosis of 4T1 cells, in the presence of IgG control or PAC-SABIs. The error bars represent the mean ± SD ( n = 3 independent experiments). c Representative 3D CLSM image reconstruction of in vitro phagocytosis of 4T1 cells (pHrodo-red + , red) by BMDMs and RAW264.7 cells (Calcein-AM, green). Three independent experiments were performed. d Representative flow cytometry plots depicting BMDM phagocytosis of 4T1 cells treated with IgG control, anti-CD24 mAb, SAMIs and PAC-SABIs. e Quantitative analysis of BMDM flow cytometry results. The error bars represent the mean ± SD ( n = 3 independent experiments; ** p < 0.01, *** p < 0.001; the p value was analyzed by a two-tailed unpaired Student’s t test). f Representative flow cytometry plots depicting RAW264.7 cell phagocytosis of 4T1 cells treated with IgG control, anti-CD24 mAb, SAMIs and PAC-SABIs. g Quantitative analysis of RAW264.7 cell flow cytometry results. The error bars represent the mean ± SD ( n = 3 independent experiments; ** p < 0.01; the p value was analyzed by a two-tailed unpaired Student’s t test). Source data are provided as a Source Data file.

Article Snippet: Treatment: anti-mouse CD24 antibody (BioXcell, #BE0360, clone M1/69), anti-human CD24 antibody (Novus Biologics, #NB100-64861, clone SN3), anti-mouse CD47 antibody (BioXcell, #BE0270, clone MIAP301), anti-mouse PD-1 antibody (BioXcell, #BE0273, clone 29F.1A12TM), anti-CSF1R antibody (BioXcell, #BE0213, clone AFS98).

Techniques: Control, In Vitro, Flow Cytometry, Two Tailed Test

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-mouse CD24 (150Nd) clone M1/69 , Fluidigm , 3150009B.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry